Integration of Filamentous Phage Xf2 DNA into Chromosomal DNA ox Xanthomonas campestris pv. oryzae.
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چکیده
منابع مشابه
Distribution of Xanthomonas oryzae pv. oryzae DNA modification systems in Asia.
The presence or absence of two DNA modification systems, XorI and XorII, in 195 strains of Xanthomonas oryzae pv. oryzae collected from different major rice-growing countries of Asia was assessed. All four possible phenotypes (XorI+ XorII+, XorI+ XorII-, XorI- XorII+ and XorI- XorII-) were detected in the population at a ratio of approximately 1:2:2:2. The XorI+ XorII+ and XorI- XorII+ phenotyp...
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Most phenolic substances of plant origin are toxic to microorganisms and they confer some degree of protection to plants against phytopathogens. Xanthomonas oryzae pv. oryzae, bacterial blight pathogen of rice (Oryza sativa) was treated with phenol (monohydroxy benzene) and its effects on the morphology and cytological changes of the bacterium were studied. Total lysis of cells occurred with 5 ...
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We have isolated and characterized a lytic double-stranded DNA Xanthomonas campestris pv. campestris bacteriophage (XTP1) capable of mediating generalized transduction. The phage transduces chromosomal markers at frequencies of 10(-5) to 10(-6) transductants per PFU. We demonstrated its genetic utility by the isolation and cotransduction of linked transposon insertions to a nonselectable locus,...
متن کاملSequence, transcriptional analysis and chromosomal location of the Xanthomonas campestris pv. campestris uvrB gene.
The uvrB gene of Xanthomonas campestris pv. campestris, a Gram-negative plant pathogenic bacterium inhabiting soil and infected plants, was cloned and sequenced. This gene has the capacity to encode a polypeptide of 673 amino acid residues with a calculated molecular mass of 75.9 kDa. Its deduced amino acid sequence shows a high degree of similarity and possesses domain conservation to those of...
متن کاملCloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae.
The ftsZ gene from Xanthomonas oryzae pv. Oryzae was amplified by PCR with the specific primers, and the recombinant plasmid pET-22b-ftsZ was constructed successfully. The FtsZ with a 6× His tag was overexpressed in a soluble form in Escherichia coli BL21 and purified through a Ni-NTA agarose column. The purified recombinant FtsZ showed a single band on SDS-PAGE with an apparent molecular mass ...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1995
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.61.127